The imperative 'out' reiterates a desperations where she is pleading for her remorse to disappear.
The usage of the word 'spot' has a metaphoric interpretation for washing the hands of blood.
'Spot' is also emblematic of the hysteria & guiltregicide has brought on her & the fact that LM now has blood dyed into her conscience.
Religious imagery- 'damned' - strikes fear into the audience as it reinforces the idea of hell and eternal suffering.
[Enter Lady Macbeth with a taper] ↝ [a5s1]
As a [taper] provides light, this is emblematic of how LM is seeking light, hope & solace in her eternal mental darkness.
Jesus was seen to be the 'light' of the world, providing salvation for mankind, therefore LM's dependancy on the light could illuminate how she is hopeful for salvation & retribution for her sins.
