Testing for proteins

• What reagent is used in the protein test?

  Biuret‘s reagent
• What does the Biuret test detect?

  The presence of amino acids or proteins
• What indicates a positive result in the Biuret test?

  A colour change from blue to purple
• What indicates a negative result in the Biuret test?

  The solution remains blue
• Is the Biuret test qualitative or quantitative?

  Qualitative
• What other method can be used to identify amino acids in a mixture?

  Chromatography
• How does chromatography separate amino acids?

  By separating them based on their mass
• Describe the test for proteins.

  1. Add biuret reagent to the sample
  2. Positive result: Blue to purple