Genetic engineering

• what is recombinant DNA Steps?

  • Restriction enzyme recognises specific sequences of DNA and cut the DNA at those points
  • ligase enzyme joins 2 pieces of DNA together
  • two different bits of DNA stuck together - recombinant DNA
• what are vectors?

  • something used to transfer DNA into cell - 2 sorts - plasmids and viruses
• how does genetic engineering work?

  • DNA you want to insert cut out with restriction enzyme - vector DNA cut open using same restriction enzyme
  • vector DNA and DNA you insert mixed with ligase enzymes
  • ligase joins 2 pieces of DNA together to produce a recombinant DNA
  • recombinant DNA transferred into other cells
  • cells now use gene you inserted to make protein you wish
• what is transgenic?

  • contain genes transferred from other species