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    Cards (9)

    • scatchard analysis
      assumption that when [L] is in great access over [P] the [L] is essentially equal to [L]TOTAL (so always want excess of ...)
    • Scatchard analysis
      straight line/ gradient, allowing us to determine the protein concentration
    • calculating KD
      *
    • Retinoic Scatchard Analysis
      free retinoic acid (ligand) binds to charcoal. Mix receptor with the acid(radioactively spiked), add charcoal pellets, centrifuge until reached equilibrium, remove pellets + free ... ) *measure amount bound
    • problems w Retinoic Scatchard analysis
      non-specific binding sites, may bind to glass
    • bound ligand/ free ligand
      slope
    • hill plot
      describes binding of n molecules of O2 to Haemoglobin

    • Hill
      Hill coefficient-n regarding Cooperativityn = 1 none, n > 1 positive, n < 1 negative
    • bohr effect 

      pH lowers due to H+being released from metabolic processess ****
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