tutorial

• What does PCR stand for?

  Polymerase Chain Reaction
• What is the primary purpose of PCR?

  To amplify specific fragments of DNA
• How can PCR be used with RNA?

  By converting RNA to DNA first
• What does qPCR allow for in PCR?

  Quantitative tracking of the reaction
• What is ELISA used for?

  To quantify the amount of protein
• What role do antibodies play in ELISA?

  They quantify the amount of protein
• What is Western blot used for?

  To quantify the amount of protein
• How does Western blot identify proteins?

  By using specific antibodies to the target protein
• What can Western blot provide in terms of quantification?

  Relative or absolute quantification
• What is immunocytochemistry?

  A technique using antibodies to detect targets
• What does mass spectrometry detect?

  Proteins using amino acid sequences
• How many proteins can mass spectrometry identify simultaneously?

  Thousands of proteins
• What can mass spectrometry predict using bioinformatics?

  Cell activity
• What does CRISPR stand for?

  Clustered Regularly Interspaced Short Palindromic Repeats
• What is the main use of CRISPR?

  To genetically engineer cells
• What does CRISPR allow for in genetic engineering?

  Precise insertion of DNA into the genome
• What are the two main applications of CRISPR?

  Gene introduction and gene knock-out
• What are viruses used for in genetic engineering?

  Highly efficient genetic alteration
• What is the difference between plasmids and viruses in genetic engineering?

  Plasmids provide less efficient alteration
• What are the differences between ELISA and immunocytochemistry?

  • ELISA quantifies protein amounts
  • Immunocytochemistry detects proteins in cells
  • ELISA uses a substrate for signal
  • Immunocytochemistry uses antibodies for visualization
• What techniques might be used to assess protein changes in response to drug treatment in the brain?

  • Mass spectrometry for overall protein changes
  • Western blot for specific protein quantification
  • ELISA for protein presence and amount
• What techniques can determine if a protein is present in the brain?

  • ELISA for quantification
  • Western blot for specific detection
  • Immunocytochemistry for localization
• What technique can provide protein expression for a limited time?

  • CRISPR for transient gene expression
  • Viral vectors for temporary protein expression
• How can you determine if a protein is expressed in response to drug treatment?

  • Western blot for protein levels
  • ELISA for quantification
  • Immunocytochemistry for localization
• What techniques can identify which cells express a protein?

  • Immunocytochemistry for localization
  • Mass spectrometry for protein identification
  • Western blot for specific detection
• What technique can be used to study gene function in the hippocampus?

  • CRISPR for gene editing
  • Western blot for protein expression
  • ELISA for protein quantification