sterilise Petri dish and agar gel
sterilise inoculating loop by flaming to kill any bacteria present
dip into microorganism and make streaks on agar gel plate
replace lid and secure with tape so no unwanted bacteria can enter and no harmful bacteria can grow
incubate and store upside down so condensation doesn't disturb organisms
incubate at 25° so no harmful bacteria can be formed
soak filter paper disks in a variety of solutions to kill any bacteria on pretri dish
use a control filter paper disk
measure clear area around soaked paper disk
