enzymes

• step 1

  put a drop of iodine solution into every well of a spotting tile
• step 2

  place a bunsen burner on a heat proof mat and a tripod and gauze over the bunsen burner.

  put a beaker of water on top of the tripod and heat the water until its 35 degrees.
• step 3

  use a syringe to add 1cm cubed of amylase solution and 1cm cubed a buffer solution with a ph of 5 to a boiling tube.

  put the boiling tube in the beaker of water for 5 minutes
• step 4

  use a different syringe to add 5cm cubed of a starch solution to the boiling tube
• step 5

  mix the contents of the boiling tube and start a stop clock
• step 6

  use a pipette to take a drop of the solutions in the boiling tube and add it to the spotting tile every 30 seconds.

  when the iodine remains browny-orange, starch is no longer present
• step 7

  repeat the experiment with buffer solutions of different ph values to see how ph affects the time taken for the starch to be broken down