microscopy

• equation:

  • image size = actual size * magnification
  • i = am
• light microscope:

  • has two lenses - objective and eyepiece lens
  • approximately a maximum magnification of x2000 and a resolving power 200nm
  • used to view tissues, cells, and large sub-cellular structures
• scanning electron microscope:

  • can view deep inside sub-cellular structures
  • slightly lower magnification than transmission
  • creates 3d images
  • needs specimen to be dead - specimen is destroyed
• transmission electron microscope:

  • can view deep inside sub-cellular structures
  • slightly higher magnification than scanning
  • creates 2d images