Preparing slides

• What materials are necessary to prepare a slide?

  • Glass slide
  • Cover slip
  • Tweezers
  • Staining solution
• What should you add to the slide after cutting the specimen?

  A drop of minimal water
• What can happen if too much water is added to the slide?

  It can blur the image
• What tool is used to handle small specimens?

  Tweezers
• What is the purpose of a cover slip?

  To cover the specimen
• What is the first material needed to prepare a slide?

  Glass slide
• What does PAS stain for?

  Carbohydrates
• What tissue does Congo Red stain?

  Amyloid
• What do differential stains do?

  Color specific cell components
• What is the name of the stain used to visualize microorganisms?

  Gram's Stain
• What is the first step in preparing a specimen?

  Cut a thin section of the tissue
• What is the name of the stain used to visualize connective muscle tissue?

  Picrosirus Red
• What is the name of the stain used to visualize amyloid deposits?

  Congo Red
• What is the name of the stain used to visualize neuronal tissue?

  Cresyl Violet
• What are the types of stains and their functions?

  • Differential stains: Color specific cell components
  • Selective stains: Highlight particular tissue types
• What tissue does Reticulin stain?

  Reticular fibers
• How should the specimen be spread on the slide?

  Evenly to form a single layer
• What do selective stains highlight?

  Particular tissue types
• When is it necessary to use a stain on the specimen?

  If necessary to highlight cell structures
• What are the key differences between the scanning electron microscope (SEM) and the transmission electron microscope (TEM) in terms of their imaging capabilities?

  • SEMs produce 3D images, while TEMs produce 2D images
  • SEMs are better for studying surface features, while TEMs are better for internal cellular structures
• What angle should the cover slip be lowered at?

  45 degree angle
• What is the object dropping a liquid droplet in the image?

  Pipette
• What is the purpose of the pipette dropping liquid onto the object in the image?

  To apply the liquid to the object for analysis or testing
• What is the purpose of the triangular prism-shaped object in the image?

  • To act as a sample or specimen for analysis or testing
• Why is it important to lower the cover slip at an angle?

  To avoid trapping air bubbles
• What tissue does Cresyl Violet stain?

  Neural tissue
• What is the purpose of using a stain on the specimen?

  To highlight cell structures
• What does the image sequence illustrate in slide preparation?

  The steps of mounting a slide properly
• How do staining techniques help in histology?

  They help differentiate structures in tissues
• What is the shape of the object the liquid is being dropped onto?

  • Triangular prism
• If the triangular prism-shaped object represents a biological sample, which type of microscope would be most appropriate to use to study its detailed structure?

  Scanning electron microscope
• Why is it important to cut a thin section of the tissue?

  To ensure only one cell layer is present
• What is the purpose of using staining solutions?

  • To produce specific colored results
  • To differentiate structures in tissues
• What are the key components of carbohydrates shown in the image?

  • Alcian Blue/PAS
  • PAS
  • Cat # 24200
• What are the key components of pigments, minerals, and granules shown in the image?

  • Rapid PTAH
  • Prussian Blue Iron
  • Fontana Masson
  • Von Kossa Method
  • Villanueva Osteochrome Bone
• Why is a staining solution used in slide preparation?

  It highlights cell structures for better viewing
• What does Oil Red O stain?

  Triglycerides
• What are the steps to properly mount a slide?

  1. Prepare the specimen with a water drop.
  2. Lower the cover slip at a 45 degree angle.
  3. Add the mounting medium and seal the edges.
• What are the key components of triglycerides and lipids shown in the image?

  • Oil Red O
  • Reticulin Fibers
  • Jones PAS-M