BIOL201 - RESPIRATORY CHAIN

• overview of mitochondrial function

  
• overview of the respiratory chain arranged according to redox potentials

  
• summary of electron transport through the respiratory chain

  
• Tools for measuring H+ pumping and electron transport

  
• Tools for measuring H+ pumping and electron transport - use of inhibitors

  
• Determining H+/electron stiochiometries

  
• Overview of complex I

  
• Mechanism for H+ pumping for complex I

  
• Updated mechanism of H+ pumping for complex I

  
• Overview of complex II

  
• Overview of complex III

  
• Mechanism of H+ pumping for complex III- The Q cycle, first oxidation

  
• the Q cycle- second oxidation

  
• Overview of H+ pumping for complex III

  
• Net result of the Q cycle

  
• the ability to split and recycle electrons from the oxidation of UQH2 is key to doubling the H+ pumped per UQH2 oxidised by complex III

  
• overview of complex IV

  
• structure of complex IV from paracoccus

  
• overview of electron transport in complex IV

  
• subunit of complex IV is arranged so as to form three pore-like structures

  
• electron transport and H+ consumption in complex IV

  
• electron transport and H+ consumption in complex IV (2)

  
• overall reaction in subunit I of complex IV for four electron transfer and production of 2 molecules of H20