prelab phytochem

• What is the first step in Dragendorff's Reagent procedure?

  Mix concentrated extract with 2M HCl
• How long should the mixture be heated in Dragendorff's Reagent procedure?

  5 minutes
• What should be added after heating in Dragendorff's Reagent procedure?

  Add 5 drops of Dragendorff reagent
• What is the first step in the Keller-Killani Test?

  Add 10 mL of 2:1 hexane:water to the extract
• What should be noted after adding H₂SO4 in the Keller-Killani Test?

  Note the color of the interface
• What is added to the portion obtained in Keller-Killani for the Liebermann-Burchard Test?

  Add 5 mL of DCM
• What is the purpose of adding anhydrous sodium sulfate in the Liebermann-Burchard Test?

  To dry the filtrate
• What is the next step after adding 3 drops of anhydride in the Liebermann-Burchard Test?

  Add 1 drop of H₂SO4
• How long should the mixture stand after adding H₂SO4 in the Liebermann-Burchard Test?

  For 1 hour
• What is the first step in the Kedde Reagent procedure?

  Add 5 mL of DCM to the Keller-Killani portion
• How long should the mixture stand after stirring in the Kedde Reagent procedure?

  Stand for 10 minutes
• What should be added after collecting the DCM layer in the Kedde Reagent procedure?

  Add 4 drops of Kedde reagent
• What is observed after adding Kedde reagent?

  Observe the color
• What is the first step in Bornträger's Test?

  Add 10 mL of distilled water to the plant extract
• What should be done after filtering in Bornträger's Test?

  Add 5 mL of benzene
• What is done after collecting the benzene layer in Bornträger's Test?

  Add 5 mL of ammonia
• What is the first step in the Bate-Smith and Metcalfe Test?

  Add 5 mL of 2:1 hexane:water to the extract
• What should be done after collecting the aqueous layer in the Bate-Smith and Metcalfe Test?

  Heat in a water bath
• What is added after heating in the Bate-Smith and Metcalfe Test?

  Add 10 mL of methanol
• How long should the mixture stand in the water bath in the Bate-Smith and Metcalfe Test?

  For 15 minutes
• What is the first step in the Froth Test?

  Add concentrated extract to distilled water
• How long should the mixture be shaken in the Froth Test?

  Shake for 30 seconds
• What should be observed after standing in the Froth Test?

  Observe for honeycomb froth
• What is the first step in the Ferric Chloride Test?

  Add 10 mL of hot distilled water
• What is added after filtering in the Ferric Chloride Test?

  Add 3 drops of ferric chloride
• What should be compared in the Ferric Chloride Test?

  Compare the color of the solution