IDENTIFICATION

• METHODS OF IDENTIFICATION?

  • antibiotic resistance
  • green fluorescent protein
  • enzyme markers
• GREEN FLUORESCENT PROTEIN?

  • gene from jellyfish producing GFP
  • gene of interest transplanted into centre of GFP gene
  • any that do not pick up gene continue to produce GFP
• ENZYME MARKERS?

  • lactase turns colourless substrate blue
  • gene of interest transplanted into lactase gene
  • any that do not pick up gene will not turn substrate blue
• ANTIBIOTIC RESISTANCE MARKER GENES?

  • plasmid containing gene for resistance to ampicillin and broken gene for resistance to tetracycline with added DNA from gene of interest
  • replica plating
  • if grows on ampicillin it contains plasmid
  • if grows on ampicillin and tetracycline it contains plasmid but no gene of interest
  • if grows on ampicillin and not tetracycline it contains plasmid and gene of interest