Popliteal fossa is a diamond-shaped intermuscular space situated at the back of the knee, most prominent when the knee joint is flexed, with boundaries laterally: biceps femoris above and lateral head of the gastrocnemius and plantaris below, medially: semimembranosus and semitendinosus above and medial head of the gastrocnemius below, floor: popliteal surface of femur, posterior ligament of the knee joint, and popliteus muscle, roof: skin, superficial fascia, and deep fascia of thigh.