The Calvin cycle uses ATP and NADPH produced during the light-dependent reactions to power the synthesis of glucose.
The membrane of a thylakoid sits inside of a chloroplast.
The thylakoid membrane loops around to form the thylakoid.
The lumen is on the inside of the thylakoid membrane.
The stroma is on the outside of the thylakoid membrane.
The thylakoid membrane is a standard membrane that is found in many organelles.
The thylakoid membrane has a phospho-bilipid layer.
Protons can cross the thylakoid membrane using the energy from electrons going to lower energy states.
The outside of the thylakoid membrane is hydrophilic because it operates well in a polar environment.
The insides of the thylakoid membrane are non-polar or hydrophobic.
Complexes span across the thylakoid membrane, including photosystem II, photosystem I, and ATP synthase.
The electrons get excited first in the chlorophyll of photosystem II.
The electrons get less and less excited as they get handed off from one complex to another complex, ending up in photosystem I.
The first place where the electrons get excited is in the chlorophyll of photosystem II.
The electrons get excited again in photosystem I.
Plastocyanin is a component of photosystem I.
Plastoquinone and cytochrome B6F complex are components of photosystem II.
Electrons are comfortable in water and in chlorophyll A, and they are even more comfortable in photosystem I.
Another photon or another set of photons coming in from 93 million miles away can excite the chlorophyll in photosystem I, which excites the excited electron, releasing energy.
The excited electron in plastoquinone transfers to the cytochrome B6F complex, which is a slightly lower energy state.
The excited electron in chlorophyll A goes to a high energy state and then transfers to the primary electron acceptor, pheophytin, which is a chlorophyll A molecule.
The excited electron in the cytochrome B6F complex transfers to the plastocyanin complex, which is a slightly lower energy state.
A photon excites electrons in chlorophyll A or other pigment molecules, which can then excite the photophorylation A directly or excite the electrons in chlorophyll A directly.
The excited electron in pheophytin is at a very high energy state and then transfers to the plastoquinone, which is a slightly lower energy state.
The excited electron in the plastocyanin complex transfers to photosystem I, which is an even lower energy state.
Pheophytin is part of the photosystem complex and the electron can jump from the chlorophyll to the pheophytin.
The electrons get handed off, the whole time that energy is being used to transfer hydrogen protons from the stroma into the lumen.
Photosystem II is where everything starts from.
The electron can still be transferred to other things and release energy.
Once the hydrogen and the electron meet, the hydrogen will be on the other side, able to freely go away again.
Through chemiosmosis, the electron eventually goes through the ATP synthase channel, turns around this part of this protein complex or enzyme complex and actually generates ATP.
The electron can drive the proton pump and eventually ends up in the NADPH at a fairly high level of energy still.
Water gets oxidized by the water oxidation on photosystem II and that electron ends up and replaces the electron in the chlorophyll.
As the electron goes down to NADPH, you are pumping protons across the membrane.
The electron wants to go into a lower energy state, so it'll rotate around, allowing the hydrogen to cross the barrier.
The electron gets transferred from one molecule to another, gets excited again, and keeps going all the way, eventually being accepted by the NAD+ to become NADPH.
In the electron transport chain video, when I talk about cellular respiration, I give a visual concept of how this actually might happen.
The electrons going from one molecule to another can help pump hydrogen through.
The electron starts off at a low energy state and the only way this happens is by energy from light.
In a gross oversimplification, the electron on a pheophytin molecule can attract a hydrogen proton to rotate around and cross the barrier.