RED BLOOD CELL INCLUSIONS

• inclusion bodies are nuclear or cytoplasmic aggregates of stainable substance, usually proteins.
• In RBC it is indication of some pathological conditions
• These bodies are never enclosed by a membrane

  and serve as storage vessels.

  Inclusion bodies
• which is a polymer of glucose, is stored as a reserve of carbohydrate and energy.

  Glycogen
• Glycogen, which is a polymer of glucose, is stored

  as a reserve of

  carbohydrate and energy
• are abnormal inclusions of iron found inside RBC on a routine blood stain.


  Pappenheimer bodies
• Formed by phagosomes that have engulfed excessive

  amounts of iron.


  Pappenheimer bodies
• basophilic erythrocytic inclusions usually located at the periphery of the cell.


  Pappenheimer bodies
• PAPPENHEIMER BODY appear as dense, blue-purple granules within the red blood cell.
• 1 or 2 in number

  PAPPENHEIMER BODY
• Cells containing Pappenheimer bodies can be confused

  with late reticulocytes but it can not take prussian blue

  stain
• PAPPENHEIMER BODY Can be visible with

  Wright or Giemsa stain
• _ is more useful for identification and

  differentiation for PAPPENHEIMER BODY

  Prussian blue
• PAPPENHEIMER BODY also stain with Romanowsky stains because of co- precipitation of ribosomes
• Pappenheimer bodies are most likely caused by accelerated red cell division or impaired hemoglobin synthesis.
• These are histopathologic findings of basophilic nuclear remnants in circulating erythrocytes.

  HOWELL-JOLLY BODIES
• Also known as “Micronucleated Reticulocytes".

  HOWELL-JOLLY BODIES
• 1 micrometer in diameter.

  HOWELL-JOLLY BODIES
• Small rounded,dense purple granules appearance.

  HOWELL-JOLLY BODIES
• Howell-Jolly bodies are composed of:

  Deoxyribonucleic acid (DNA)

  Nuclear remnants
• Howell-Jolly bodies are found in:

  Circulating erythrocytes

  Erythroid precursors in bone marrow
• Howell-Jolly bodies are associated with:

  Rapid or abnormal RBCs formation
• HOWELL-JOLLY BODIES

  Peripheral blood smear

  Wright stain (methylene blue + eosin)

  Remnants of nucleus (DNA) observes
• They are inclusions within red blood cells composed of denatured hemoglobin.

  HEINZ BODY
• HEINZ BODY They are inclusions within red blood cells

  composed of

  denatured hemoglobin
• Heinz bodies appear as small round inclusions

  within the red cell body.
• Heinz bodies are formed by damage and

  denaturing to the hemoglobin component of red

  blood cells, most commonly by ??

  oxidative stress

  genetic abnormalities in hemoglobin
• They are literally loop, rings or figure 8 types

  structure that are located with in the cytoplasm of

  RBC.

  CABOT RING
• They are typically colored red purple under the Wright stain

  CABOT RING
• They are quite rare

  CABOT RING
• They are simple microtubular remnants of mitotic

  tubules that are involve in mitosis

  CABOT RING
• CABOT RING

  Rare condition

  Check the morphology of red blood cell using a

  blood film
• coarse, dust-like dots represent ribosomes and polyribosomes (RNA in precipitated aggregates).

  BASOPHILIC STIPPLING
• BASOPHILIC STIPPLING: Cells containing these granules are often _, so they may be difficult to distinguish.

  polychromatophilic
• It is seen whenever erythropoiesis is rushed, or in

  lead poisoning.

  BASOPHILIC STIPPLING
• Basophilic stippling is also referred to as punctuate

  "dots" basophilia
• DISEASES ASSOCIATED with Fine basophilic stippling

  polychromatophilia
• DISEASES ASSOCIATED with Coarse “rough” basophilic stippling

  lead poisoning, and thalassemia

  impaired hemoglobin synthesis
• These dark red, hexagonal crystals are shaped like

  bacilli rods and stain pink-red, are thick.

  HEMOGLOBIN C CRYSTALS
• sometimes can be confusing to new techs who may

  mistake them for bacteria.

  HEMOGLOBIN C CRYSTALS