DNA fragments

• What is the purpose of gel electrophoresis?

  To separate DNA fragments of different sizes
• How are DNA fragments prepared for gel electrophoresis?

  They are cut with restriction endonuclease enzymes
• What medium is used in gel electrophoresis?

  Agarose gel
• What charge does DNA have in gel electrophoresis?

  Negative charge
• In which direction do DNA fragments move during gel electrophoresis?

  Towards the anode
• Why do different DNA fragments create bands at different heights?

  They move at different rates based on size
• What is the role of gel electrophoresis in DNA profiling?

  • Separates DNA fragments by size
  • Creates distinct bands for analysis
  • Used for identification and comparison
• What are the risks associated with gel electrophoresis?

  Significant risks with the practical process
• Why might simulations be used instead of actual gel electrophoresis?

  Due to risks and expensive equipment
• Where can you find a detailed explanation of gel electrophoresis?

  Video from Addgene
• What is the website mentioned for further information on gel electrophoresis?

  www.pmt.education