Organism development

• What methods are used to record changes in average temperature?

  Ice cores, peat bogs, dendrochronology
• What causes climate change according to the study material?

  The greenhouse effect from increased greenhouse gases
• How does the greenhouse effect influence temperature?

  It increases absorption of infrared light
• What are some effects of climate change listed in the material?

  Melting polar ice caps and natural disasters
• What changes to organisms can result from climate change?

  Changes to morphology, number, and distribution
• What equipment is needed for the brine shrimp experiment?

  • Brine shrimp egg cysts
  • Sea salt
  • De-chlorinated water
  • Salt water
  • Beakers
  • Water bath
  • Stirring rod
  • Magnifying glass
  • Forceps
  • Pipette
  • Bright light
  • Refrigerator
  • Graph paper
• What is the method for hatching brine shrimp eggs?

  1. Dissolve 2g of sea salt in 100 cm of dechlorinated water.
  2. Use graph paper to collect eggs.
  3. Incubate at 5-35 degrees Celsius for 24 hours.
  4. Count hatched larvae after shining a bright light.
  5. Repeat at various temperatures.
• What is the optimum temperature for hatching brine shrimp?

  About 25 degrees Celsius
• What happens to shrimp hatching as temperature increases beyond the optimum?

  The number of shrimp hatched decreases
• How does climate change affect brine shrimp development?

  Increased sea water temperature decreases development
• How can the brine shrimp experiment be modified for seedlings?

  • Measure effect of climate change on seedling growth rate.
  • Optimum temperature varies by species.
  • Growth increases up to an optimum temperature, then decreases.
  • Similar vulnerability to climate change as brine shrimp.
• What are the risks associated with the brine shrimp experiment?

  • Broken glass: Cuts; handle glassware carefully.
  • Hot liquids: Scalding; use tongs and eye protection.
  • Biohazard: Contamination; use disinfectant and wash hands.
• What should be done in case of a broken glass hazard?

  Elevate cuts and seek medical assistance
• What safety precaution should be taken with hot liquids?

  Use tongs and wear eye protection
• What should be done after handling biohazards?

  Wash hands with soap and use disinfectant
• What type of graph should be plotted in the experiment?

  • Plot temperature against number of hatched larvae.