Gene cloning + transfer

• How does PCR/invitro cloning work?

  • Temp of DNA increased to 95C⁰ to break hydrogen bonds, leaving 2 single strands of DNA
  • Temp decreased to 55C⁰ so primers can attach to each single strand of DNA
  • DNA polymerase joins free nucleotides by phosphodiester bonds
  • Temp drops to 72C⁰ so hydrogen bonds can form between the new complimentary strand and the single strand
• What are primers?

  • Short single stranded chains of DNA that are complimentary to a longer DNA strand