3rd Test unit 10-12

• • is a measurement of the hydrogen ion (H+) concentration solution
  • is an indication of the acidity or alkalinity of solution
  • 0-14?

  pH
• What is pH7?

  Neutral
• Hydrogen ion (H+) represent?

  acidity
• Hydroxal ion (OH-) represent?

  Alkaline or basic pH
• Accurate ph readings measured by a pH metre with an attached pH electrode or probe?

  pH meters
• What is the end of the probe?

  Glass bulb
• • Senses the differences in the electrical charges between the inside of the glass bulb/electrode and outside of the glass bulb/electrode
  • so it creates current?

  pH meters
• What is the difference charges between outside and inside surface of the glass?

  electrical potential
• What is the equation to calculate pH?

  pH = -log[H+]
• What [H+] means?

  concentration of hydrogen ions
• Connecting or directing a machine to reflect accurate value (could be biological cabinet, pipette, pH meter, centrifuge) ?

  Calibration
• Various pH we would use to calibrate a pH metre?

  4.0, 7.0, 10.0
• • pH occurs when you have hydrogen ions (H+)
  • pH values below 7 are?
  • The lower, the pH the more?

  Acidic
• • pH of occurs when you have hydroxyl ions (OH-)
  • pH values above 7 are?
  • The higher, the pH more?

  Alkaline
• Is 7.2 alkaline or acidic?

  alkaline
• Is baking soda, alkaline or acidic?

  alkaline
• pH of 11 is it hydroxyl or hydrogen ion?

  Hydroxyl ion OH-
• pH of 6 is it hydroxyl or hydrogen ion?

  Hydrogen ion H+
• If you receive a biohazard bag with blood tubes in it and labels is not attached what would you do?

  reject and discard it / call a physician
• If you received a biohazard bag with CSF/cerebrospinal fluid in it and label is not attached what would you do?

  CSF is invasive/irritable so call a physician or nurse
• • Light blue
  • coagulant
  • 3 to 4 inversion?

  citrate tube
• • Red/gold
  • no anticoagulant
  • 5 times inversion?

  serum tube
• • Dark green/light green
  • coagulant
  • 8 to 10 times inversion?

  heparin tube
• • Purple/pink
  • no anticoagulant
  • 8 to 10 times inversion?

  EDTA tube
• • Gray tube
  • no anticoagulant
  • 8 to 10 times inversion?

  fluoride (glucose) tube
• If hemolysis occur, in vitro, what colour of plasma would be?

  Pink or red
• If hemolysis occur invivo what colour of plasma would be?

  dark yellow called Icterus sample
• Icter caused by?

  Bilirubin
• is the destruction of red blood cells by the breakdown of their membrane?

  Hemolysis
• If there is hemolysis in the sample which analyte is falsely elevated?

  potassium
• Invivo mean?

  in vein/body
• Invitro mean?

  in tubes/glass
• What temperature we set incubator for microbiology/bacteria?

  37 degrees Celsius
• What temperature we set for yeast and fungi?

  30 degrees Celsius
• Temperature range/normal for incubator?

  35 to 39 degree celsius
• Fridge, formal optimal temperature?

  4 to 7 degree celsius
• If the power goes out the digital display that in the freezer will stop working what measurement you will use?

  independent measurement
• Consequences of underfilled EDTA tube/lavender?

  can affect cell count or shrinks the red blood cells
• • _____ must be refrigerated within the first hour of collection if you're not testing it yet
  • if it left or not be put in the fridge, bacteria will grow multiple?

  Urine
• CNS / C&S stands for?

  culture and sensitivity