effect of ph on enzyemes

• what does amalyse do

  • amalyse breaks down starch into glucose
• what doe starch do to iodine

  • starch turns iodine to blue
• experiment

  • use a place only one drop of iodine in a spotting dwell
  • prepare different ph of buffer solution and amalyse and mix together
  • transfer starch in solution and mix the contents together as well
  • use continous sampling via a clock for a set time period and use a pipette to place a drop of the mixed solution into spotting dwell
  • record how long it takes for iodine to turn blue
• what is the control variable In the experiment

  • volume of amylase solution and volume of starch solution
• what is the independent variable

  • the pH of buffer solution
• what is the dependant variable

  • the time it takes for iodine to remain orange/starch to be broken don
• what is the quickest pH for starch to be broken down mean

  • it's the Optum pH
• why does some pH take longer for starch to be broken down then othersor if there's no change in iodine colour

  amalyse active site denatures meaning no enzyeme substrate complex can be formed as starch can't bind to the active site