Enzymology

• Enzymes used in Tanzer-Gilvarg

  1st reaction: Creatine kinase

  2nd reaction: Pyruvate kinase

  3rd reaction: Lactate dehydrogenase
• Enzymes used in Oliver-Rosalski

  1st reaction: Creatine kinase

  2nd reaction: Hexokinase

  3rd reaction: G6PD
• LD isoenzymes found in Liver and Skeletal muscles: LD4 and LD5
• LD isoenzyme found in lungs, lymphocytes, spleen, and pancreas: LD3
• LD isoenzyme found in Heart, RBC, and Renal cortex: LD1 and LD2
• Wacker

  Lactate + NAD + LDH → Pyruvate + NADH + H
• Wroblewski La Due

  Pyruvate + NADH + H + LDH → Lactate + NAD