SDS-PAGE uses sodium dodecyl sulphate (SDS), an anionic detergent that disrupts protein secondary, tertiary, and quaternary structures, giving proteins a net-negative charge.
Reducing conditions in SDS PAGE reduce disulphide bonds and covalent bonds within a protein.
Discontinuous PAGE involves a gel with two components: a stacking gel with large pores and a separating (main) gel that separates polypeptides.
Development in a sealed container is a method of TLC.
