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Notes
Gram -
Genus Brucella
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Cards (30)
What is the Gram stain result for Brucella?
Gram
-ve
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What type of bacteria is Brucella in terms of catalase?
Catalase
+ve
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What is the shape of Brucella bacteria?
Short Rods
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Why does Brucella not have flagella?
It is a
non-motile
bacterium
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What type of aerobe is Brucella?
Facultative Anaerobe
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What does it mean for Brucella to be fastidious?
It requires specific nutrients to grow
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Does Brucella have a capsule?
Rarely
Thin Capsule
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What does it mean for Brucella to be facultative intracellular?
It can live inside
macrophages
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What does Brucella need added to its culture medium?
Protein
addition like
blood
or
serum
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What is the primary reservoir for Brucella?
Farm Animals
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How is Brucella transmitted?
Direct
or
Indirect
Contact
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What is a key virulence factor of Brucella?
Lipopolysaccharide
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What does the lipopolysaccharide in Brucella trigger?
Macrophage
and tissue factor
activations
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What is the function of the Type IV Secretion System in Brucella?
Facilitates
protein transport
and
DNA transformation
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What does superoxide dismutase do in Brucella?
Scavenges
oxygen
radicals
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What does urease do in Brucella?
Converts
urea
to
ammonia
and
CO2
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How does urease affect pH in Brucella infections?
Increases
pH
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Why can Brucella survive intracellularly in macrophages?
Antibodies
cannot reach them
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What are somatic thermostable agents in Brucella?
Agents
A & M
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What are the clinical manifestations of Brucellosis?
Flu-like symptoms
Night sweats
High
undulant fever
Painful lymphadenopathy
Localized infections:
arthralgias
, endocarditis, osteomyelitis
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How does Brucella penetrate the body?
Through
intact
skin
or
mucosa
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What happens to Brucella after it enters the lymph nodes?
Enters
reticuloendothelial system
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What type of granuloma can form due to Brucella infection?
Granuloma
in liver, spleen, kidneys
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What specimens are used for microbiological diagnosis of Brucella?
Blood
Bone marrow
CSF
Lymph node aspirates
Tissue materials
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What is the growth characteristic of Brucella in culture?
Slow
and
insufficient growth
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When is serological investigation for Brucella done?
10
days after
infection
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What is the principle of the ELISA test for Brucella?
Detects
antigen-antibody
binding
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What is the first-line treatment for Brucellosis?
Doxycycline
+
Rifampin
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What is the second-line treatment for Brucellosis?
Doxycycline
+
Streptomycin
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What type of vaccine is used for Brucellosis prevention?
Alive Vaccine
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