Genus Brucella

Cards (30)

  • What is the Gram stain result for Brucella?
    Gram -ve
  • What type of bacteria is Brucella in terms of catalase?
    Catalase +ve
  • What is the shape of Brucella bacteria?
    Short Rods
  • Why does Brucella not have flagella?
    It is a non-motile bacterium
  • What type of aerobe is Brucella?
    Facultative Anaerobe
  • What does it mean for Brucella to be fastidious?
    It requires specific nutrients to grow
  • Does Brucella have a capsule?
    Rarely Thin Capsule
  • What does it mean for Brucella to be facultative intracellular?
    It can live inside macrophages
  • What does Brucella need added to its culture medium?
    Protein addition like blood or serum
  • What is the primary reservoir for Brucella?
    Farm Animals
  • How is Brucella transmitted?
    Direct or Indirect Contact
  • What is a key virulence factor of Brucella?
    Lipopolysaccharide
  • What does the lipopolysaccharide in Brucella trigger?
    Macrophage and tissue factor activations
  • What is the function of the Type IV Secretion System in Brucella?
    Facilitates protein transport and DNA transformation
  • What does superoxide dismutase do in Brucella?
    Scavenges oxygen radicals
  • What does urease do in Brucella?
    Converts urea to ammonia and CO2
  • How does urease affect pH in Brucella infections?
    Increases pH
  • Why can Brucella survive intracellularly in macrophages?
    Antibodies cannot reach them
  • What are somatic thermostable agents in Brucella?
    Agents A & M
  • What are the clinical manifestations of Brucellosis?
    • Flu-like symptoms
    • Night sweats
    • High undulant fever
    • Painful lymphadenopathy
    • Localized infections: arthralgias, endocarditis, osteomyelitis
  • How does Brucella penetrate the body?
    Through intact skin or mucosa
  • What happens to Brucella after it enters the lymph nodes?
    Enters reticuloendothelial system
  • What type of granuloma can form due to Brucella infection?
    Granuloma in liver, spleen, kidneys
  • What specimens are used for microbiological diagnosis of Brucella?
    • Blood
    • Bone marrow
    • CSF
    • Lymph node aspirates
    • Tissue materials
  • What is the growth characteristic of Brucella in culture?
    Slow and insufficient growth
  • When is serological investigation for Brucella done?
    10 days after infection
  • What is the principle of the ELISA test for Brucella?
    Detects antigen-antibody binding
  • What is the first-line treatment for Brucellosis?
    Doxycycline + Rifampin
  • What is the second-line treatment for Brucellosis?
    Doxycycline + Streptomycin
  • What type of vaccine is used for Brucellosis prevention?
    Alive Vaccine