The method for investigating the effect of light intensity on transpiration using a bubble potometer involves cutting a shoot underwater to prevent air entering the xylem and placing it in a tube, setting up a lamp 10cm from the leaf, allowing the plant to adapt to the new environment for 5 minutes, recording the starting location of the air bubble, leaving for 30 minutes, recording the end location of the air bubble, changing the light intensity, resetting the bubble by opening the tap below the reservoir, and repeating the experiment.