Microscopy 1 what is

• What does microscopy refer to?

  The use of microscopes
• What are the two main types of microscopes discussed?

  Light microscopes
• What is the function of the base of a light microscope?

  It supports the entire microscope
• What connects the base to the top of a light microscope?

  The arm
• What type of light source is used in the discussed microscope?

  A mirror reflecting room light
• Where is the microscope slide placed?

  On the stage
• How many objective lenses are typically found in a light microscope?

  Three objective lenses
• What are the possible magnifications of the objective lenses?

  10x, 20x, and 50x
• What is the function of the eyepiece lens?

  It has a fixed magnification
• What are the two types of focusing knobs on a microscope?

  Coarse and fine focusing knobs
• What does the term 'object' refer to in microscopy?

  The real sample being observed
• What does the term 'image' refer to in microscopy?

  The visual representation seen through the microscope
• How does light travel through a light microscope?

  It reflects off the mirror, through the object, and lenses
• What is magnification in microscopy?

  How many times larger the image is than the object
• What is the equation for magnification?

  Magnification = image size / object size
• If an image is 1000 times larger than the object, what is the magnification?

  Times one thousand
• What is resolution in microscopy?

  The shortest distance between distinguishable points
• How does resolution affect image clarity?

  Higher resolution means more detail and less blur
• What can be inferred about two images with the same magnification but different resolutions?

  The one with lower resolution appears blurrier
• What happens to the level of detail as resolution increases?

  More details become visible
• What are the main components of a light microscope?

  • Base
  • Arm
  • Light source (mirror or lamp)
  • Stage
  • Objective lenses (3 types)
  • Eyepiece lens
  • Body tube
  • Coarse and fine focusing knobs
• What is the process of light travel in a light microscope?

  1. Light hits the mirror
  2. Light reflects through the object
  3. Light passes through objective lenses
  4. Light goes through the eyepiece lens
  5. Light enters the eye
• What are the differences between object and image in microscopy?

  • Object: The real sample being observed
  • Image: The visual representation seen through the microscope