PCR

• PCR reactions amplify a specific segment of DNA
• PCR does NOT contain fluorescent dye, so it is not visible to the naked eye
• DNA polymerase is an enzyme which collects free nucleotides and bonds them together to make new DNA
• Primers are used to signal to DNA polymerase where to start copying along the template DNA strand.
• Buffers maintain a constant PH level
• 3 steps of PCR reaction are : Denaturation, annealing, and extension
• PCR reactions involves a process of heating, cooling and then heating again
• In denaturation, high temperature separates the DNA into two single strands
• After each PCR reaction, the number of DNA molecules doubles.
• PCR amplifies DNA, but does not tell us the sequence of it.