meselsohn and stahl experiment

• What did Meselson and Stahl do?

  they proved semi conservative DNA replication
• what is the first step of the meselson and stahl experiment

  e.coli is grown in a medium containing amino acids and nitrogen 15 a heavy isotope
• what happends in the meselson and stahl experiment after the e.coli is grown

  the DNA is extracted and centrifuged
• what happens after the e.coli is primarily extracted and centrifuged

  the bacteria is washed and then transferred to a medium with amino acids and a lighter 14 nitrogen isotope
• what happens in the meselson and stahl experiment after the bacteria is transferred to a lighter isotope


  it is allowed to divide once and then is extracted and centrifuged
• what happens after the bacteria is extracted and centrifuged for a seccond time

  the bacteria is allowed to divide again in the 14 nitrogen medium and then is centrifuged
• what is a nitrogen medium

  this is a growth medium for bacteria
• what is the end product of the Meselson and stahl experiment

  4 newly synthesised dna stands , 2 being 14n/14n and 2 being 14n/15n strands