CPAC 2.1

    Cards (7)

    • Aim?
      Investigate membrane structure and effect of temperature on membrane permeability
    • Independent variable?
      Temperature of water baths (0°C, 10°C, 20°C, 30°C, 40°C, 50°C )
    • Dependent variable?
      Percentage transmission of light through the solutions
    • Control Variables?
      Volume of distilled water for each boiling tube (10cm3cm^3)
      Time left in water baths (30 mins)
      Size of beetroot (1cm) - cylindrical
      Age or source of beetroot
      same colorimeter with same settings
      Same volume of beetroot solution added to cuvette (2cm3cm^3)
    • Control?
      Keep a boiling tube containing beetroot and distilled water in room temperature and use colorimeter.
    • Why beetroot?
      Betalain is a pigment in the vacuole of a beetroot cell. As membrane permeability increases, the pigment begins to leak which can be measured, using a colorimeter.
    • Conclusions?
      As temperature increases, the percentage transmission also increases, indicating that membrane permeability increases.
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