4.5.2 Mass spectrometry (MS)

    Cards (26)

    • What is mass spectrometry (MS) used to identify and determine?
      Molecules and molecular mass
    • Arrange the steps of mass spectrometry in the correct order:
      1️⃣ Ionization
      2️⃣ Acceleration
      3️⃣ Deflection
      4️⃣ Detection
    • The detection step in mass spectrometry measures the abundance of ions at each m/z value to create a mass spectrum.

      True
    • What type of fragmentation occurs in Electrospray Ionization (ESI)?
      Minimal fragmentation
    • The mass spectrometer produces a mass spectrum
    • Electrospray Ionization (ESI) causes minimal fragmentation of molecules.

      True
    • A mass spectrometer produces a spectrum by measuring the abundance of ions at each m/z value.
    • Steps in interpreting mass spectrometry (MS) data
      1️⃣ Identify the molecular ion peak
      2️⃣ Analyze fragment ions
      3️⃣ Determine molecular mass
      4️⃣ Deduce structural information
    • The molecular ion peak's m/z value indicates the molecule's molecular mass.
    • Match the MS data feature with its description:
      Molecular Ion Peak ↔️ Highest mass peak
      Fragment Ions ↔️ Series of lower mass peaks
    • MS is used to determine the molecular mass of a compound by analyzing the molecular ion peak.
    • Match the MS concept with its explanation:
      m/z ↔️ Ratio of mass to charge
      Deflection ↔️ Determined by m/z
      Molecular Ion Peak ↔️ Represents parent ion
    • What are two applications of MS in organic chemistry?
      Determining molecular mass and identifying unknown substances
    • The first step in mass spectrometry is ionization
    • How are ions deflected in the magnetic field of a mass spectrometer?
      By their mass-to-charge ratio
    • Electron Impact (EI) is a gas-phase ionization technique that uses high-energy electrons
    • Match the ionization technique with its key characteristic:
      Electron Impact (EI) ↔️ High fragmentation
      Electrospray Ionization (ESI) ↔️ Minimal fragmentation
    • What type of ionization is used in Electron Impact (EI)?
      Gas phase
    • Electron Impact (EI) is a gas-phase ionization technique where molecules are bombarded with high-energy electrons to produce a fragmentation pattern.
    • Match the MS characteristic with its explanation:
      m/z ↔️ Ratio of ion's mass to charge
      Deflection ↔️ Lighter ions deflect more
      Importance ↔️ Identifies structure and mass
    • The m/z value determines how much an ion is deflected in the magnetic field of the mass spectrometer.
      True
    • What does the molecular ion peak represent in MS data?
      Parent ion (M+)
    • Fragment ions provide insights into the molecule's structure by revealing specific structural components.

      True
    • What is one application of MS in organic chemistry?
      Determining molecular mass
    • Fragmentation patterns in MS can reveal the arrangement of functional groups in a molecule.
      True
    • The molecular ion peak's m/z value indicates the molecule's molecular mass.