1.1.5 Microscopy

• Why is microscopy essential in cell biology?

  Visualizing and studying cells
• Electron microscopes can visualize living cells

  False
• A light microscope uses visible light to produce an image.
• Arrange the parts of a light microscope in the order light passes through them:

  1️⃣ Illumination System

  2️⃣ Condenser Lens

  3️⃣ Specimen

  4️⃣ Objective Lens

  5️⃣ Eyepiece Lens
• The objective lens of a light microscope forms a magnified real
• What does the condenser lens in a light microscope do?

  Focuses light
• Fixation is a sample preparation technique that chemically preserves the cell structure
• Sectioning involves cutting the sample into thin slices for detailed observation.


  True
• Which sample preparation technique chemically preserves cell structure?

  Fixation
• Electron microscopes can achieve magnification up to 1,000,000x
• Which type of microscope requires extensive sample preparation?

  Electron microscope
• What type of beam does an electron microscope use to create an image?

  Electron beam
• The illumination system in a light microscope provides the light necessary for image formation.


  True
• The illumination system of a light microscope provides the light that passes through the specimen and into the objective
• Fixation in sample preparation involves chemically preserving the cell structure to prevent changes
• Sample preparation techniques help overcome the limitation of light microscopes, such as the inability to view living cells.


  True
• Match the part of a light microscope with its function:

  Objective Lens ↔️ Forms a magnified real image

  Eyepiece Lens ↔️ Further magnifies the real image

  Condenser Lens ↔️ Focuses light on the specimen

  Illumination System ↔️ Provides light for the specimen
• The formula for total magnification is: Total Magnification = (Magnification of Objective Lens) × (Magnification of Eyepiece
• The maximum magnification of a light microscope is typically around 1000x
• Order the types of microscopes from least to most expensive:

  1️⃣ Light Microscope

  2️⃣ Confocal Microscope

  3️⃣ Electron Microscope
• Match the microscope type with its characteristics:

  Light Microscope ↔️ Lower resolution and simple preparation

  Electron Microscope ↔️ Higher resolution and extensive preparation
• Match the type of microscope with its characteristic:

  Light Microscope ↔️ Up to 1000x magnification

  Electron Microscope ↔️ Requires extensive sample preparation
• What type of microscope can visualize very small cellular structures but cannot view living cells?

  Electron microscope
• Order the key parts of a light microscope from top to bottom:

  1️⃣ Eyepiece Lens

  2️⃣ Objective Lens

  3️⃣ Condenser Lens

  4️⃣ Illumination System
• The eyepiece lens magnifies the real image formed by the objective lens to produce the final image.
• Match the sample preparation technique with its description:

  Fixation ↔️ Chemically preserving cell structure

  Staining ↔️ Adding dyes to highlight structures

  Sectioning ↔️ Cutting sample into thin slices

  Mounting ↔️ Placing sample on a slide
• One advantage of a light microscope is that it can view living cells.
• Light microscopes cannot resolve very small cellular structures due to their limited magnification.


  True
• Light microscopes can resolve very small cellular structures.

  False
• The TEM is used for visualizing internal cellular structures
• The TEM requires thin sections of the sample for creating a high-resolution image
• Electron microscopes offer higher magnification and resolution compared to light microscopes.


  True
• Why is microscopy essential in cell biology?

  Visualizing cell structure
• What type of light is used in a light microscope?

  Visible light
• The light microscope can magnify up to 1000x
• Light microscopes are suitable for observing living cells.


  True
• Electron microscopes can visualize living cells.

  False
• What is the function of the objective lens in a light microscope?

  Forms a magnified real image
• The illumination system provides light for the objective lens.


  True
• Staining involves adding dyes