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Nucleic Acids
Semi-conservative DNA replication
Semi-conservative mechanism
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Cards (109)
What is the semi-conservative mechanism?
DNA replication
creating two
identical
copies
In what way does the dispersive mechanism differ from the semi-conservative mechanism?
Dispersive has mixed old and new
segments
What does each new DNA molecule consist of in the semi-conservative mechanism?
One old
strand
and one new strand
What is the significance of the semi-conservative mechanism?
It ensures accurate replication of
genetic information
How does the structure of new DNA in the conservative mechanism differ from the semi-conservative mechanism?
Conservative has two new strands, semi-conservative has one
old strand
What is the name of the enzyme that joins the Okazaki fragments on the lagging strand?
Ligase
How do the components of DNA replication work together?
They replicate DNA effectively in
sequence
What does ligase do during DNA replication?
It joins
Okazaki fragments
on the
lagging strand
How is DNA replication similar to building with Legos?
Split the original DNA (big Lego tower) in half
Add new
nucleotides
(new Legos) to each half
Create two identical DNA strands (complete towers)
What is the significance of the coordination among helicase, RNA primers, DNA polymerase, and ligase in DNA replication?
It ensures
effective
and
accurate
DNA replication
How does the replication of the leading and lagging strands differ?
Leading strand: Continuous synthesis in the
5' to 3'
direction
Lagging strand: Discontinuous synthesis in the 5' to 3' direction, forming
Okazaki fragments
that are later joined by
ligase
What is the function of DNA polymerase?
It adds new
nucleotides
to the growing DNA strand
What is a replication fork?
The point where DNA
unwinds
during replication
What is the name of the enzyme that replicates DNA?
Primase
What are the key components involved in DNA replication?
Helicase
: unwinds the DNA double helix
RNA primers
: mark where replication begins
DNA polymerase
: adds new nucleotides
Ligase
: joins
Okazaki fragments
What happens after the big Lego tower is split in half?
New Legos
are
added
to
each
half
How does the semi-conservative mechanism ensure DNA fidelity?
By using one
original
and one
new strand
What is a template strand in DNA replication?
The original strand guiding new strand
synthesis
What is the significance of the double helix structure of DNA?
It allows for efficient storage and
replication
of
genetic
information
What is the structure of new DNA in the semi-conservative mechanism?
One
old
strand paired with one new strand
What is the role of DNA polymerase?
It
synthesizes
new DNA strands
How does the semi-conservative mechanism differ from conservative and dispersive mechanisms?
It uses one
old strand
and one new strand
What happens to the DNA strands after helicase unwinds them?
They separate into single strands for
replication
What is the significance of having both old and new Legos in the new towers?
It ensures genetic continuity and variation
What do RNA primers do in DNA replication?
They provide a starting point for DNA
synthesis
What do the two new Lego towers represent in DNA replication?
Two
identical
DNA strands formed
What do RNA primers do in DNA replication?
They mark where replication should
begin
What is the purpose of the RNA primers laid down by primase during DNA replication?
To provide a starting point for
DNA polymerase
to
synthesize
new DNA strands
What are the characteristics of the three DNA replication mechanisms?
Semi-conservative
:
One old strand paired with one new strand
Conservative
:
Two completely new
strands
, original intact
Dispersive
:
Each strand is a mix of old and new fragments
If we have a DNA strand and helicase unwinds it, what is the next step?
Add
RNA primers
to initiate synthesis
What are the three main components of a nucleotide?
Phosphate group
Sugar (
deoxyribose
)
Nitrogenous base
How does the process of DNA replication resemble magic?
It creates
identical
copies from one original
Why is accurate replication of the DNA sequence important?
To maintain
genetic information
integrity
How does the addition of new nucleotides occur during DNA replication?
Each nucleotide adds to its
specific
complementary
base
What structure is essential for DNA replication?
Double helix
structure
If
the sequence of base pairs in a DNA strand is ATCGAT, what would the
complementary
sequence
be
?
TAGCTA
What analogy is used to describe RNA primers' function?
Like the first
Lego
brick to start building
What analogy is used to describe helicase's function?
Like a
zipper
that opens two sides
What is the name of the enzyme that plays a role in elongation during DNA replication?
DNA polymerase III
What stabilizes the specific pairing of DNA bases?
Hydrogen bonds
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