HISTOPATH/MTLAWS PE

• b. Carbohydrates


  The PAS reaction will demonstrate fungi, because the cell wall contains:

  a. Lipids

  b. Carbohydrates

  c. Reducing substances

  d. Argyrophilic protein
• a. Fite


  The best stain for the demonstration of Mycobacterium leprae is the:

  a. Fite

  b. PAS

  c. Kinyoun

  d. Gram
• c. Liposarcomas


  The oil red O stain might be used to demonstrate:

  a. Rhabdomyosarcomas

  b. Leiomyosarcomas

  c. Liposarcomas

  d. Adenocarcinomas
• c. Frozen


  The oil red O stain requires which of the following sections?

  a. Paraffin

  b. Celloidin

  c. Frozen

  d. Plastic
• a. Verhoeff


  Which of the following methods best demonstrates elastic tissue?

  a. Verhoeff

  b. Silver impreganation

  c. Gomori trichrome

  d. PAS
• a. Congo red


  Amyloid can be demonstrated with:

  a. Congo red

  b. Mayer mucicarmine

  c. Cresyl echt violet

  d. Alcian blue
• c. PAS with and without diastase


  Glycogen is best demonstrated by the use of:

  a. Crystal violet

  b. Mayer mucicarmine

  c. PAS with and without diastase

  d. Alcian blue with and without hyaluronidase
• a. Acid alcohol


  Sections for special stains have been accidentally stained with hematoxylin. To remove the hematoxylin, place the sections in:

  a. Acid alcohol

  b. Dilute ammonia

  c. Lithium carbonate

  d. Isopropyl alcohol
• a. DNA only


  The Feulgen reaction demonstrates:

  a. DNA only

  b. RNA only

  c. Both DNA and RNA

  d. Phosphoric acid groups
• b. Weak acid


  Differentiating in the H&E stain is an example of using:

  a. Excess mordant

  b. Weak acid

  c. Oxidizers

  d. Buffers
• c. RNA


  Which of the following is stained rose by the methyl green-pyronin (MGP) technique?

  a. Heterochromatin

  b. DNA

  c. RNA

  d. Golgi apparatus
• b. Make nuclear staining more specific


  Acetic acid is added to Harris hematoxylin to:

  a. Keep heterochromatin from staining

  b. Make nuclear staining more specific

  c. Ripen the hematoxylin

  d. Form a dye lake
• a. Iron


  The mordant in Weigert hematoxylin is:

  a. Iron

  b. Aluminum

  c. Mercury

  d. Tungsten
• c. Feulgen reaction


  DNA can be demonstrated with:

  a. Eosin

  b. Pyronin

  c. Feulgen reaction

  d. Fast green
• a. Form hematein


  Mercuric oxide (or sodium iodate) is used in Harris hematoxylin to:

  a. Form hematein

  b. Prevent oxidation

  c. Serve as the mordant

  d. Stabilize the solution
• c. Lake


  The combination of a dye and a mordant is called a/an:

  a. Base

  b. Accelerator

  c. Lake

  d. Buffer
• c. Link tissue constituents more closely to the dye


  Mordants are used to:

  a. Change the refractive index of the tissue

  b. Help differentiate stains

  c. Link tissue constituents more closely to the dye

  d. Oxidize staining solutions
• c. Differentiation in acid-alcohol


  The most important step in regressive hematoxylin staining is:

  a. Postmordanting in picric acid

  b. Use of hematoxylin containing acetic acid

  c. Differentiation in acid-alcohol

  d. Washing in water after the hematoxylin
• b. Hematein


  The active staining ingredient in ripened hematoxylin solutions is:

  a. Hematin

  b. Hematein

  c. Hematoxylin

  d. Hemosiderin
• b. Oxidation


  Ripening of hematoxylin is a process of:

  a. Hydrolysis

  b. Oxidation

  c. Mordanting

  d. Reduction
• c. Nuclei


  Harris hematoxylin is used on tissue sections to stain:

  a. Fat

  b. Glycogen

  c. Nuclei

  d. Cytoplasm
• c. Nonionizing radiation


  The microwave oven creates heat in staining solutions by:

  a. Convection

  b. Conduction

  c. Nonionizing radiation

  d. Electrolytic action
• b. 400

  
  

  Important:

  Total magnification (TM)

  = magnification of objective x magnification of ocular

  = 10 x 40

  = 400

  When using a microscope with a x10 ocular and a x40 objective, the total magnification is approximately:

  a. 100

  b. 400

  c. 1,000

  d. 4,000
• c. 60C


  The temperature of the oven used to maintain a supply of melted paraffin for embedding tissue should be about:

  a. 43C

  b. 43F

  c. 60C

  d. 60F
• c. Temperature of the infiltrating paraffin


  During microtomy, it is noted that most of the tissue is very hard and shrunken. One of the first things to check to prevent its happening in the future is the:

  a. Presence of water in the clearing agent

  b. pH of the fixative

  c. Temperature of the infiltrating paraffin

  d. Freshness of the reagents on the processor
• d. Water-soluble wax

  The dehydration and clearing steps can be omitted when using:

  a. Celloidin

  b. Epoxy resin

  c. Glycol methacrylate

  d. Water-soluble wax
• a. Dehydration


  The process of removing water from tissue is called:

  a. Dehydration

  b. Reduction

  c. Oxidation

  d. Clearing
• c. Ethanol

  Which of the following chemicals is NOT a clearing agent?

  a. Chloroform

  b. Dioxane

  c. Ethanol

  d. Xylene
• a. Benzene


  A reagent that CANNOT be used for dehydrating tissue is:

  a. Benzene

  b. Absolute alcohol

  c. Dioxane

  d. Acetone
• b. For both dehydrating and clearing tissues

  Dioxane is a reagent that can be used:

  a. For both fixing and dehydrating tissues

  b. For both dehydrating and clearing tissues

  c. In very small volume ratios

  d. For long periods without changing
• b. Dehydrant and paraffin

  A clearing agent for use in processing tissues for paraffin embedding must be miscible with the:

  a. Fixative and paraffin

  b. Dehydrant and paraffin

  c. Fixative and dehydrant

  d. Paraffin and water
• d. Alcohol


  Which of the following is a dehydrating agent?

  a. Formalin

  b. Xylene

  c. Benzene

  d. Alcohol
• a. Prolonged fixation

  Which of the following may cause tissue to become overhardened?

  a. Prolonged fixation

  b. Abbreviated fixation

  c. Inadequate dehydration

  d. Incomplete clearing
• a. Fixative


  The first and most important procedure in the preparation of a tissue for microscopic examination is the choice of:

  a. Fixative

  b. Dehydrating agent

  c. Clearing agent

  d. Staining technique
• a. Chloroform

  Carnoy fluid is prepared with acetic acid, alcohol, and:

  a. Chloroform

  b. Formalin

  c. Acetone

  d. Osmium tetroxide
• b. 900 mL

  To prepare a 10% solution of formalin, which of the following amounts of water should be added to 100 mL of stock formaldehyde:

  a. 1,000 mL

  b. 900 mL

  c. 450 mL

  d. 10 mL
• c. Alcoholic picric acid


  Formalin pigment may be removed from tissue by:

  a. Running water

  b. Alcoholic iodine

  c. Alcoholic picric acid

  d. Potassium permanganate
• c. 37 to 40% formaldehyde

  
  

  Important:

  Commercial stock solution: 37 to 40% formaldehyde

  As fixing fluid: 10% formalin

  Commercial stock formaldehyde solutions contain:

  a. 4% formaldehyde

  b. 10% formaldehyde

  c. 37 to 40% formaldehyde

  d. 98 to 100% formaldehyde
• d. Iodine


  Precipitate left in tissues that have been fixed in solutions containing mercuric chloride may be removed by immersion in:

  a. Running water

  b. Sodium thiosulfate

  c. Weak ammonia water

  d. Iodine
• b. Helly

  Which of the following fixatives contains formalin, potassium dichromate, and mercuric chloride?

  a. Zenker

  b. Helly

  c. Carnoy

  d. Orth