CC - Specimen collection & Reagent Preparation

• ULTRACENTRIFUGATION


  reference method for LIPOPROTEIN QUANTITATION
• UItracentrifuges

  High speed centrifuge, Chamber is refrigerated to counter heat due to friction
• 52° angle


  fixed angle for Fixed-angle or Angle- head centrifuge
• Fixed-angle or Angle- head centrifuge

  angled compartments for the tubes, small particles sediment more rapidly
• approx. 3000rpm


  Speed for Horizontal or swinging bucket centrifuge
• Horizontal or swinging bucket Centrifuge


  centrifuge that is horizontal when spinning; vertical when not moving
• Electronic balances


  uses electromagnetic force to counterbalance the weighed sample's mass
• Analytic balances


  mechanical analytic balance is also known as a substitution balance
• Dessicator


  airtight chamber used with dessicants
• Dessicant


  hygroscopic substances used as excellent drying substance
• Burets


  dispense a particular volume of liquid during titration
• Teflon-tipped

  Positive - displacement pipet plunger
• Positive - displacement pipets

  Uses a glass capillary tip, No carryover, tips are reusable, useful if reagent reacts to PLASTIC, procedures require WASHING or FLUSHING STEP between samples
• Air-displacement pipets

  a pipette where there is an AIR CUSHION in between piston and sample, safer to use, uses a disposable polypropylene tip
• Automatic macropipets/ micropipets


  most routinely used pipets
• Pasteur Pipets


  Pipets that do not have calibration marks and are used to transfer solutions or biologic fluids without consideration of a specific volume
• Ostwald-Folin pipette


  a pipette that transfer fluids having a viscosity greater than water, blow-out
• volumetric pipet


  a pipette that dispense aqueous solutions, self-draining
• Micropipette


  a pipette with a pipetting capability of less than 1 mL
• Mohr Pipet


  no graduation, self-draining, tip should NOT be allowed to touch the vessel while draining
• serological pipette

  graduation marks to the tip. A BLOWOUT PIPET
• Self-draining pipet


  pipet where contents are drained by gravity
• Blowout pipet


  pipet with continuous etched ring near top of pipet
• To deliver (TD) pipet

  pipet that dispense volume or entire liquid indicated
• To contain (TC) pipet

  Pipet that holds a particular volume, DOESN'T DISPENSE exact volume
• 3 types of thermometers

  1. liquid in glass2. electronic thermometer /thermistor probe3. digital thermometer
• Type I water


  Type of water that has the most stringent requirements and generally suitable for routine lab use
• Secondary Standard reference materials

  LOWER PURITY with concentration determined by comparison with a primary standard
• Primary standard reference materials

  HIGHLY PURIFIED CHEMICAL that can be measured directly to produce substance of exact known concentration and purity
• Technical / Commercial Grade


  Reagent grade that should NEVER BE USED in the clinical laboratory​
• United States Pharmacopeia (USP)/ National Formulary (NF)


  Reagent grade that used to manufacture drugs
• Chemically Pure (CP) or Pure Grade reagents


  Reagent grade that is NOT RECOMMENDED for use unless further purification or a reagent blank is included
• Ultrapure reagents


  require extremely PURE CHEMICALS
• Analytic Reagent (AR)


  established by the American Chemical Society (ACS), labels on reagents state the: ACTUAL IMPURITIES
• Urine, CSF, paracentesis fluids (pleural, pericardial, peritoneal), Amniotic fluid


  Other samples analyzed in CC lab
• Amniotic fluid


  used to assess fetal lung maturity ( L/ S ratio), congenital diseases,hemolytic diseases, genetic defects and gestational age
• POC glucose


  highest volume of POC test
• POINT-OF- CARE TESTING (POCT)


  Lab test that is performed outside the central or core lab and generally at the site of clinical care or close to the patient
• ASAP collections


  do it at a possible time
• STAT means

  Immediately, in latin: statim, given the highest priority