Testing for sugars

• What equipment is used to grind food for testing?

  Pestle and mortar
• What safety equipment should be worn during the experiment?

  Safety goggles
• How much of the test material solution should be placed in the boiling tube?

  2 cm³
• What reagent is added to the test material solution?

  Benedict's reagent
• How long should the boiling tube be placed in the water bath?

  5 minutes
• What does a color change in Benedict's reagent indicate?

  Presence of reducing sugars
• What color does Benedict's reagent turn if reducing sugar is present?

  Green, yellow, orange, or red
• What are the steps to test for reducing sugars using Benedict's solution?

  1. Grind food and mix with distilled water.
  2. Filter the mixture.
  3. Place 2 cm³ of solution in a boiling tube.
  4. Add 2 cm³ of Benedict's reagent.
  5. Heat in a water bath for 5 minutes.
  6. Observe color change.