independent assortment / random segregation , of (homologous) chromosomes in , metaphase I / meiosis I homologous chromosomes , have different alleles / come from different parents ; produces large number of allele combination. crossing over / in , prophase I / meiosis I chromatids will have new combination of alleles. mutation changes the (DNA) nucleotide/ base, sequence. random, mating / fusion of gametes/ fertilisation gametes are not genetically identical produces large number of (allele) combinations