Methods of studying cells

• The equation for magnification is as followers:

  Magnification = Size of image/Size of real object
• The magnification of an object is how many times bigger the image is when compared to the object.
• Resolution is the minimum distance apart that two objects can be in order for them to appear as separate items.
• The process of cell fractionation is as follows:

  1. Tissue is cut up and kept in a cold, buffered solution.
  2. Cut up tissue is further broken up in a homogeniser.
  3. Homogenised tissue is spun in a ultracentrifuge at a low speed for 10 minutes.
  4. The result of being spun at a slow speed is a supernatent and a sediment.