Each test tube is individually set up: Test Tube A: moist cotton wool and placed in warm environment (hot water bath set at appropriate temperature), Test Tube B: dry cotton wool and placed in warm environment (hot water bath set at appropriate temperature), Test Tube C: moist cotton wool and is placed in cold environment (bucket of ice), Test Tube D: cotton wool soaked in boiled Water that is cooled off, and layered with Oil on top.