Lecture #07: Glycolysis & Respiration

• The enzyme used in step 1 is hexokinase

  
• Hexokinase adds an inorganic phosphate to glucose, forming glucose 6-phosphate

  
• The enzyme used in step 2 is phosphoglucoisomerase

  
• Phosphoglucoisomerase transforms glucose 6-phosphate into its isomer, fructose 6-phosphate

  
• The enzyme is used in step 3 is

  A) Phosphofructokinase

  
• Phosphofructokinase adds an inorganic phosphate to Fructose 6-phosphate, forming:

  A) Fructose 1,6-bisphosphate

  
• The enzyme used in step 4 is:

  A) Aldolase

  
• Aldolase splits Fructose 1,6-bisphosphate into 2 molecules:

  A) Glyceraldehyde 3-phosphate (G3P)

  B) Dihydroxyacetone phosphate (DHAP)

  
• The enzyme used in step 5 to transform DHAP to G3P is:

  A) Isomerase

  
• What is the rate-limiting step in Glycolysis: Energy Investment?

  Phosphofructokinase
• The enzyme used in step 6 is:

  A) Triose phosphate dehydrogenase

  
• Triose phosphate dehydrogenase reduces NAD+ & adds an inorganic phosphate to G3P, forming:

  A) 1,3-Bisphosphoglycerate

  
• The enzyme used in step 7 is:

  A) Phosphoglycerokinase

  
• Phosphoglycerokinase removes an inorganic phosphate from 1,3-Bisphosphoglycerate, making ATP via substrate-level phosphorylation; this forms:

  A) 3-Phosphoglycerate

  
• The enzyme used in step 8 is:

  A) Phosphoglyceromutase

  
• Phosphoglyceromutase transforms 3-Phosphoglycerate into its isomer:

  A) 2-Phosphoglycerate

  
• The enzyme used in step 9 is:

  A) Enolase

  
• Enolase transforms 2-Phosphoglycerate into:

  A) Phosphoenolpyruvate (PEP)

  
• ___ is very unstable & has high free energy.

  Phosphoenolpyruvate (PEP)
• The enzyme used in step 10 is:

  A) Pyruvate kinase

  
• Pyruvate kinase removes an inorganic phosphate from PEP to form ___; this exergonic reaction is coupled to make ATP via substrate-level phosophorylation

  A) Pyruvate

  
• Fill in the enzymes and intermediate products for Glycolysis: Energy Investment Phase I

  A) Hexokinase

  B) Glucose 6-Phosphate

  C) Phosphoglucoisomerase

  D) Fructose 6-Phosphate

  
• Fill in the enzymes and intermediate products for Glycolysis: Energy Investment Phase II

  A) Phosphofructokinase

  B) Fructose 1,6-Bisphosphate

  C) Aldolase

  D) Glyceraldehyde 3-Phosphate (G3P)

  E) Dihydroxyacetone phosphate (DHAP)

  F) Isomerase

  
• Fill in the enzymes and intermediate products for Glycolysis: Energy PAYOFF Phase I

  A) Triose phosphate dehydrogenase

  B) 1,3-Bisphosphoglycerate

  C) Phosphoglycerokinase

  D) 3-Phosphoglycerate

  
• Fill in the enzymes and intermediate products for Glycolysis: Energy PAYOFF Phase II

  A) Phosphoglyceromutase

  B) 2-Phosphoglycerate

  C) Enolase

  D) Phosphoenolpyruvate (PEP)

  E) Pyruvate kinase

  F) Pyruvate