MLSP LAB

• Gold or Tiger Red/Black Top

  • Contains spray-coated silica, which is the active substance responsible for the immediate formation of a clot.
  • Contains a polymer gel separator that separates the serum from the clotted cells after centrifugation.
• Red top tubes may contain additive solution (Silica particles), but most of the case it does not.
• Orange top contains a silicon-coated interior with thrombin, which is the active substance responsible for the immediate formation of a clot.
• Gold, orange, red (container w/ coagulants) are used in clinical chemistry and for the testing of serum for infectious diseases.
• Purple or Lavender Top: May either contain K3EDTA (Liquid) or K2EDTA (spray particles).
• Purple top is called the “Anticoagulant of Choice”
• Purple top is used in hematologic analysis (CBC) and can also be used in immunohematology, such as blood typing and screening
• What top is Generally preferred for molecular techniques?

  Purple or Lavender
• Green Top contains either sodium heparin or lithium heparin.
• other than Gold or tiger red, which has a polymer gel an top?

  Green
• Heparin is the active substance that inhibits clot formation by irreversibly binding with antithrombin III and greatly accelerates the rate at which it inactivates coagulation enzymes.
• “Anticoagulant of choice” for STAT plasma analysis?

  Green
• Green top is used for plasma analysis in clinical chemistry
• Gray/ Grey Top may either contain both potassium oxalate and sodium fluoride or Na2EDTA and sodium fluoride
• Sodium fluoride is the active substance that inhibits glycolytic enzymes present in the sample.
• what are the anticoagulants in gray top?

  oxalate and EDTA
• Used in glucose determination in plasma.?

  Gray top
• Invertsion of light blue top?

  3-4
• Light Blue Top - Contains 3.2 % buffered sodium citrate with concentrations of 0.105 M (glass) or 0.109 M (plastic).
• In other cases, Light Blue Top may also contain citrate, theophylline, adenosine, and dipyridamole
• The action of EDTA why it inhibits clot formation because it chelates calcium
• Citrate chelates ionized calcium
• What top is used for coagulation studies in plasma (aPTT & PT)?

  Light Blue
• Black Top contains? buffered sodium citrate.
• Black top is primarily used in hematology, especially in the Westergren erythrocyte sedimentation rate determination
• Royal Blue Top May either contain a clot activator (Glass) or anticoagulant (Plastic) which separates the serum or plasma from the cellular constituents of blood.
• Royal Blue top - It is used in testing blood for trace elements within 4 hours after extraction and It can also be used in nutritional-chemistry.
• Special stopper formulation provides low levels of trace elements, what top?

  Royal Blue
• Light yellow blood culture bottles contain sodium polyanethol sulfonate which are used in microbiology, especially in blood culture
• In some instances, yellow tops may contain acid citrate dextrose (ACD) which is used in blood bank studies, HLA phenotyping, and DNA and paternity testing.
• Pink top Contains K2EDTA.
• Pink Top: It may be used in whole blood analysis in hematology, but it is primarily used in immunohematology testing like crossmatching and blood typing.
• Tan Top: May either contain sodium heparin or K2EDTA as the anticoagulant.
• Specialized blood collection tubes which do not contain any traces of lead?

  Tan top
• The order of draw for microtainers are as follows:

  1. Capillary Blood Gases
  2. EDTA Tubes
  3. Other Additive Tubes (Heparin or Oxalate/Fluoride)
  4. Serum Tubes (with or without gel separator)
• Orange top is the choice for STAT serum analysis
• Green top is for STAT plasma analysis