Microscope Slide Preparation

Cards (20)

  • What is the first step in preparing a slide with onion cells?
    Add a drop of water
  • Why do you add water to the slide when preparing onion cells?
    To provide a medium for the specimen
  • What tool is used to peel off a thin layer of onion?
    Tweezers
  • What should you do after placing the onion layer on the slide?
    Add a drop of iodine solution
  • What is the purpose of adding iodine to the onion cells?
    To highlight the cells for better visibility
  • How should you position the coverslip on the slide?
    Stand it on the edge and lower it gently
  • What is the first step in using a light microscope to observe a slide?
    Clip the slide onto the stage
  • Why should you start with the lowest-powered objective lens?
    To locate the specimen easily
  • What should you do after moving the stage up with the coarse adjustment knob?
    Look through the eyepiece and adjust
  • How do you achieve a clear image after focusing?
    Use the fine adjustment knob
  • What should you do to see more detail in the specimen?
    Switch to a higher-powered lens
  • What is the first step in drawing your observations from the microscope?
    Use a sharp pencil
  • What should you avoid adding to your drawing?
    Color or shading
  • Why is it important to draw subcellular structures in proportion?
    To accurately represent the specimen's features
  • What must be included in your drawing besides the image?
    A title and the magnification used
  • How should you label important features in your drawing?
    With straight, uncrossed lines
  • How much space should your drawing occupy?
    At least half of the available space
  • What are the steps to prepare a slide with onion cells?
    1. Add a drop of water to the slide.
    2. Extract a thin layer of onion.
    3. Position the layer in the water.
    4. Stain with iodine solution.
    5. Cover with a coverslip.
  • What are the steps to observe a slide under a light microscope?
    1. Clip the slide onto the stage.
    2. Choose the lowest-powered objective lens.
    3. Use the coarse adjustment knob to focus.
    4. Switch to a higher-powered lens for detail.
  • What are the steps to draw observations from a microscope?
    1. Use a sharp pencil.
    2. Make the drawing large and clear.
    3. Avoid color or shading.
    4. Draw structures in proportion.
    5. Include a title and magnification.
    6. Label features with straight lines.