Required Practical - Microscopy

• Light Microscope Experiment

  • Put a thin sample of tissue (e.g. onion epidermis) onto a microscope slide.
  • Add a few drops of a suitable stain/dye (e.g. iodine).
  • Place a coverslip on top of the tissue and place the slide onto the microscope stage.
  • Use the objective lens with the lowest magnification, and focus on the sample.
  • Increase the magnification and refocus to see different features of the cell.
• When using a light microscope, use the lowest power objective lens, and focus on the sample. Increase the power and then refocus.
• We can observe (see) structures within cells (sub-cellular structures) using microscopes.