Required pracs

• What is the aim of the chlorophyll chromatography practical?

  To investigate different pigments in leaves
• What does chromatography help to reveal about leaf pigments?

  It shows the combination of different pigments
• How many closely related pigments does chlorophyll typically consist of?

  Five closely related pigments
• Why do different pigments appear as different colors?

  They absorb and reflect different wavelengths of light
• How does the environment affect the pigments in plants?

  Different environments lead to different pigment combinations
• What is the hypothesis regarding pigments in different species of leaves?

  The combination of pigments differs by environment
• How can you extract pigment using the TLC method?

  Crush the leaf in a solvent
• What is the first step in the chromatography method?

  Extract the pigment from the leaf
• What is the purpose of the origin line in chromatography?

  To mark where the pigment is applied
• Why should the solvent be below the origin line?

  To prevent washing the pigment off the paper
• What should you do after applying the pigment to the chromatography paper?

  Let the drop completely dry
• What diameter should the pigment dot be approximately?

  Three to five millimeters
• Why is it important to keep the chromatography paper vertical?

  To ensure pigments move straight up the paper
• What happens to the pigments during the chromatography process?

  More soluble pigments move further up the paper
• What should you do immediately after removing the chromatography paper?

  Draw a line where the solvent reached
• Why is it necessary to draw a line at the solvent front?

  To calculate RF values accurately
• How is the RF value calculated?

  Distance moved by pigment / distance moved by solvent
• Why do we measure from the middle of the pigment mark?

  To standardize measurements for fair comparisons
• What does the RF value indicate?

  It helps identify which pigments are present
• How can you compare pigments from two different leaves?

  By comparing their chromatograms and RF values
• What factors might affect pigment differences in plants?

  Canopy cover and light duration
• Why must the origin line be drawn in pencil?

  Pens contain ink that can interfere
• Why should you use forceps to handle chromatography paper?

  To avoid contamination from fingers
• What happens to the pigments over time after chromatography?

  The colors may fade quickly
• What is the significance of comparing chromatograms from different species?

  To determine if they contain the same pigments
• What might be a reason for different pigments in plants from different environments?

  Adaptation to absorb different light wavelengths
• What is the role of the solvent in chromatography?

  To dissolve and carry the pigments up the paper
• Why is it important to repeat the chromatography method for a second leaf?

  To compare pigment presence between species
• What is the purpose of the chromatography experiment in biology?

  To study the diversity of leaf pigments
• What can be concluded if two leaves have different RF values?

  They likely contain different pigments
• What is the main focus of the video on chlorophyll chromatography?

  To explain the chromatography technique
• What is the significance of the chromatogram in the experiment?

  It visually represents pigment separation
• How does the chromatography process help in identifying pigments?

  By comparing RF values to known standards
• What should be done if the chromatography paper is not straight?

  It may cause inaccurate results
• Why is it important to use a ruler when measuring distances in chromatography?

  To ensure accurate and consistent measurements
• What could happen if the chromatography paper touches the container walls?

  It may lead to sideways pigment movement
• What is the role of the chlorophyll in plants?

  To absorb light energy for photosynthesis
• What is the expected outcome of the chromatography experiment?

  Visible separation of different pigments
• How does the chromatography technique contribute to our understanding of plant biology?

  It reveals the diversity of pigments in plants
• What factors could influence the results of the chromatography experiment?

  Solvent type and environmental conditions