INTESTINAL AND UROGENITAL FLAGELLATES

• Pathogenesis of Dientameoba Fragilis

  Non Pathogenic
• Habitat of Dientamoeba Fragilis

  lumen of the caecum & upper colon
• Mode of Infection of Dientamoeba Fragilis

  Ingestion
• Laboratory Identification of Dientamoeba Fragilis

  direct fecal smear (wet

  and stained)
• Specimen source of Dientameba

  Fragilis


  Feces
• Characteristic of Dientamoba Fragilis


  Similar to active amoeba trophozoite
• Size:small; _______; narrower size: ____

  3 – 22 um, 5 – 12 um
• the nucleus of the Dientamoeba Fragilis can be _____

  rosette-shaped

  can be mononucleated or binucleated,
• The cytoplasm of Dientameoba Fragilis may contain ______ with bacteria, flagella extends from the cell wall and

  resembles like pseudopod

  food vacuoles
• Mobility of Dientamobe Fragilis


  actively motile with progressive

  movement
• Characterized by its minute size

  Dientamoeba Fragilis
• classified as an amoeba-like flagellates

  Dientamoeba Fragilis
• Who discovered Dientamoeba Fragilis

  Pepps & Dobell (1918)
• Stain used in Dientameoba Fragilis

  Giemsa, Trichrome, Iron hematoxylin
• Can be seen in the eggs of E. vermicularis

  Dientamoeba Fragilis
• Geographical Distribution of Giardia Lamblia

  Cosmopolitan, pocket of Cosmopolitan
• Pathogenesis of Gardia Lamblia

  Giardiasis
• Serological Diagnosis of Giardia Lamblia

  Agglutination Test,

  Fluorescent Antibody, Elisa
• Habitat of Giardia Lamblia 


  small intestine
• Infected Form of Giardia mature quadrinucleated cyst
• Mode of Infection of Gardia Ingestion, sexual contact
• Laboratory Identification of Giardia Fecalysis (wet and

  stained smear)
• Specimen Source of Gardia Lamblia:

  Feces, Duodenal Aspirates
• Size of trophozoite stage of gardia

  amblia

  9to20u
• Shape of Gardia Lamblia 


  pear-shaped; sucking disc; “Wry little face”
• • Dorsal Surface: _____
  •  Ventral Surface: _____

  Convex, Concave
• • Flagella: _ Anterior:_  Ventral:_  Caudal:_

  8,4,2,2
• • give rise to the flagella; located between the anterior portion of the nuclei

  Kinestosomes
• • interior portion of the flagella; transverse the cytoplasm before emerging: the two causal axonemes divide the body longitudinally into halves

  axonemes
•   largecurved;dark-stained

  • Medium Bodies:
• • support or energy production

  parabasal bodies
• like a falling leaf


  Mobility of the gardia lamblia
• cystic size pf gardia

  Size:8to14u
• shape of cystic stage of gardia lamblia

  ovoid to ellipsoidal
• number of nuclues in cystic stage of gardia lamblia

  2 to 4 u
• appearance of cystic stage of gardia lamblia

  remnants of flagella; kinetosomes,

  axonemes, and median bodies;
• • Inflammation of the mucosal cells of the smallintestine

  Giardiasis

  •  The symptoms probably result from
• • Cause alterations such as: 
  • Villousflattening Crypthypertrophy
  • Villousflattening Crypthypertrophy
• • Cramping abdominal pain with diarrhea

  acute cases
• • Steatorrhea


  chronic cases