Results and Discussions

• • Extract made of root part of C. sativa also did show stimulatory effect to shoot length in extract concentrations of 50%
  • Bioassay revealed that for extract of the root of C. sativa there were no statistically significant deviations between values of lettuce seed GP, RGP, MGT, GI, VI from control values. For variant of 25, 75 and 100% of extract from the root parts of C. sativa
• • Duncan test showed statistically significant difference (P≤0.05) between the most germination indices of lettuce seed only in the case of the extract made of the shoot parts of C. sativa in concentration of 75 and 100%
• Results:

  • Extracts made from shoot parts in concentrations of 75 and 100% showed significant effects on germination indices and seedling growth of lettuce
  • Extract made of root part of cannabis showed stimulatory effect to shoot and seminal root length of lettuce seedlings in extract concentrations of 50, 75 and 100%
  • The structure of plasma membrane might have become denatured by the phytotoxins present in shoot extract of C. sativa when the seeds were soaked in extract
• • Contents of biologically active compounds in shoot and root parts of C. sativa are probably different both qualitatively and quantitatively
  • Higher content of allelochemicals present in extract of shoot parts of C. sativa may be responsible for the inhibition of metabolic activities of lettuce in the laboratory bioassay tests
• Discussion:

  • The inhibitory effect of different concentrations of shoot extract of C. sativa on seed germination might be due to imbalance in metabolism regulated by various enzyme activities
  • The phytotoxins present in shoot extracts of C. sativa might have arrested the protease, α-amylase activity resulting in inhibition of protein and starch breakdown which reduces the germination process
  • Allelochemicals are known to inhibit the metabolic processes i.e. cellular respiratory ability of the target plant and energy transfer which is responsible for ATP synthesis
• The results showed that the proposed method is effective in predicting the performance of different types of materials.