how can bacteria be engineered to produce human insulin?
1. a plasmid (a loop of DNA) is removed from a bacterium.
2. the insulin gene is cut out of a human chromosome using a restriction enzyme. restriction enzymes recognise specific sequences of DNA and cut the DNA at these points. the cut leaves one of the DNA strands with unpaired bases — this is called a 'sticky end'
3. the plasmid is cut open using the same restriction enzyme - leaving the same sticky ends
4. the plasmid and the human insulin gene are mixed together
5. ligase (an enzyme) is added. this joins the sticky ends together to produce recombinant DNA (two different bits of DNA stuck together)
6. the recombinant DNA is inserted into a bacterium
7. the modified bacterium is grown in a vat under controlled conditions. you end up with millions of bacteria that produce insulin.the insulin can be harvested and purified to treat people with diabetes