Cell Biology: Microscopy

Cards (10)

  • What is the equation for magnification?

    Magnification = Image height / Object height
  • What is the image?

    The image that we see when we look down the microscope
  • What is the object?
    The real object or sample that you're looking at
  • What is the resolution?The shortest distance between two points on an object that can still be distinguished as two separate entities
    • A measure of how detailed the image is
  • What are the components of a light microscope?

    The components are:
    A) Mirror
    B) Condenser
    C) Stage Clip
    D) Objective Lens
    E) Eyepiece
    F) Course Focus
    G) Fine Focus
    H) Arm
    I) Stage
  • What are the advantages of a light microscope? 

    Easy to use
    Relatively cheap
  • What are the disadvantages of a light microscope?

    Limited resolution and magnification (resolution is limited to 0.2 micrometers).
    Relies on light
    Any details less than 0.2 micrometers will appear blurry
  • What are the advantages of an electron microscope?
    Uses electrons instead of light
    Maximum resolution is 0.1 nanometers (around 2000 times better than a light microscope, so it can be used to study sub - cellular structures
  • What are the disadvantages of electron microscopes?

    Very expensive
    Hard to use so it is only used by scientists in labs
  • What are the units of conversion?

    1 m = 100 cm = 1000mm
    1 cm = 10 mm
    1 mm = 1000 μm
    1 μm = 1000nm