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Topic 1: Cell Biology
Cell Biology: Microscopy
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Cards (10)
What is the
equation
for
magnification
?
Magnification
=
Image height
/
Object height
What is the
image
?
The
image
that we
see
when we look down the
microscope
What is the object?
The
real
object or
sample
that you're looking at
What is the
resolution
?The
shortest distance
between
two
points on an object that can still be
distinguished
as
two separate entities
A
measure
of how
detailed
the image is
What are the
components
of a
light microscope
?
The components are:
A)
Mirror
B)
Condenser
C)
Stage Clip
D)
Objective Lens
E)
Eyepiece
F)
Course Focus
G)
Fine Focus
H)
Arm
I)
Stage
9
What are the
advantages
of a
light microscope
?
Easy
to
use
Relatively
cheap
What are the
disadvantages
of a
light microscope
?
Limited resolution
and
magnification
(resolution is limited to
0.2 micrometers
).
Relies on
light
Any details less than
0.2 micrometers
will appear
blurry
What are the advantages of an electron microscope?
Uses
electrons
instead of
light
Maximum resolution is
0.1 nanometers
(around
2000
times better than a light microscope, so it can be used to study
sub
-
cellular
structures
What are the
disadvantages
of electron microscopes?
Very
expensive
Hard
to use so it is only used by
scientists
in
labs
What are the units of conversion?
1
m = 100 cm = 1000mm
1 cm = 10 mm
1 mm = 1000 μm
1 μm = 1000nm