Forensic palynology is the study of pollen, spores and other acid-resistant microscopic plant bodies to prove or disprove a relationship between objects, people and places in criminal and civil cases
Forensic palynology is particularly useful in cases where there is suspected movement of evidence or where a crime has occurred in a location with distinct plant species
Flowering plants are known as angiosperms
Insect-Pollinated Flower:
Presence of petals, scent, nectar
Colourfulpetals, scent, nectar
Anthers within flower
Stigma within flower
Small quantities of larger, sticky sculpted pollen
Formation of a Pollen Grain (Male Gamete):
Pollen grains are formed in the anther by mitosis and meiosis
The tapetum provides nutrients to the developing pollen grains and produces a waterproof coat for the pollen grain
Inside each pollen grain, the haploid nucleus undergoes mitosis to produce two nuclei: a generative nucleus and a pollen tube nucleus
The pollen cell wall (exine) is tough and resistant to chemicals
Pollen is resistant to UV radiation, important for wind pollination
Female gamete (n):
The ovule contains the female gamete and is formed in the ovary by mitosis and meiosis
The megaspore mother cell in the nucellus undergoes meiosis to produce four haploid megaspores
One megaspore undergoes three mitotic divisions to produce eight haploid nuclei within the embryo sac of the ovule
Embryo Sac Development:
The 8 haploid nuclei inside each embryo sac include one female gamete, two polar nuclei, two synergids and three antipodal cells
Pollination:
Pollination is the transfer of pollen grains from the anther to the stigma
Self-pollination: transfer of pollen grains from an anther to a stigma of the same plant
Cross-pollination: transfer of pollen grains from an anther to a stigma of a different plant of the same species
Advantages of self-pollination: only one parent needed, can help maintain well-adapted genotypes
Disadvantages of self-pollination: reduces genetic variation, increases the chance of genetic diseases
Advantages of cross-pollination: increases genetic variation for species adaptation
Cross Pollination:
Stamen and stigma ripen at different times
The anther is below the stigma
Genetic/chemical incompatibility
Separate male and female plants
Preventing cross-pollination during plant reproduction experiments: enclose or isolate the flower in a bag
Fertilisation:
When a compatible pollen grain lands on a stigma, the stigma secretes a sugary solution
The pollen tube enters the embryo sac through the micropyle
Both male gametes are involved in separate fertilisation events
Double Fertilisation:
One male gamete fuses with the female gamete to produce a diploid zygote
The second male gamete fuses with the two polar nuclei to form a triploid primary endosperm nucleus
Internal fertilisation in the female involves the secretion of digestive enzymes to digest a path for the male gamete to reach the female gamete
Double fertilisation occurs in plants
Pollen tube formation in plants / acrosome reaction in humans
Male gamete is able to swim in humans / pollen seed
Mitosis forms a diploid embryo, consisting of plumule (developing shoot), radicle (developing root) and one or two cotyledons
Endosperm tissue is an important food storage tissue in cereal grains like wheat & maize
The testa develops into a fruit wall enclosing the seeds
Seeds with one cotyledon are called monocotyledons, e.g. maize & wheat
Seeds with two cotyledons are called dicotyledons, e.g. broad beans
Maize is a monocotyledon so only has one cotyledon
Broad beans are dicotyledons, they have two cotyledons, with the embryo lying between them
Broad bean seeds use the cotyledons as food storage tissue and are non-endospermic
Maize is endospermic
Seeds have evolved as a survival strategy for a terrestrial mode of life (living on land)
The testa is chemically resistant and provides physical protection for seeds
Endosperm / cotyledons provide a supply of nutrients until photosynthesis can occur
Dormant seeds have a low metabolic rate to survive over winter and germinate in spring/summer
Seeds with low water content can survive dry conditions
Fruits are important in seed dispersal as they are eaten by animals and the seeds pass through the digestive system for dispersal
Seeds can be dispersed by water, wind, or animals
Germination requires water, suitable temperature, and oxygen
In endospermic seeds like maize, gibberellin is involved in germination
Starch and proteins in seeds are broken down into soluble products by enzymes like amylase and proteases
Soluble products are transported in the plumule and radicle via phloem
Plumule grows towards light and against gravity, radicle grows away from light and towards gravity
Tapetum provides nutrients and regulatory molecules to developing pollen grains
Pollen tube nucleus contains genetic information for digesting a path through the style
Generative nucleus undergoes mitosis to form two male nuclei (gametes)