LBYBI13 Long Exam 1 Reviewer
Cards (162)
- What are the five (5) principles of microscopy?
- The ability of the lens to show two adjacent objects as discrete entities
- The microscope resolution is dependent on what factors?
- Defined as the function of the diameter of the objective lens in relation to its focal length
- The bending power of light passing through air from the glass slide to the objective lens
- For efficient magnification and resolution
- Refers to objectives that can be changed with minimal or no refocusing.
- The difference in light intensity between the specimen image and the adjacent background relative to the overall background intensity.
- Give four (4) examples of a light microscope
- A three-dimensional imaging microscopy that uses a polarizer to create two distinct beams of polarized light. Gives structures such as endospores, vacuoles, and granules a three-dimensional appearance.
- A three-dimensional imaging microscopy that uses a computerized microscope coupled with a laser source. Computer can focus the laser on single layers of the specimen. Resolution is 0.1µm
- A microscope that uses electrons instead of photons to image cells and structures
- Give two (2) types of Electron microscope
- A type of electron microscope where electromagnets function as lenses. System operates in a vacuum. High magnification and resolution (0.2 nm). Visualized structures at the molecular level. Specimen must be very thin (20-60 nm) and be stained
- A type of Electron microscope where the specimen is coated with a thin film of heavy metal (e.g. gold). An electron beams scans the object. Scattered electrons are collected by a detector and an image is produced. Even very large specimens can be observed. Magnification range of 15x - 100,000x
- Composed of nutrients used for the growth and identification of microorganisms.
- In solid media, how many percent of agar is added to the nutrient components as a solidifying agent?
- In semi-solid media, how many percent of agar is added to the nutrient components as a solidifying agent?
- In liquid media, how many percent of agar is added to the nutrient components as a solidifying agent?
- A culture media for the cultivation of non-fastidious microorganisms.
- Give an example of a simple media
- A culture media that contains additional nutrient sources for the growth of fastidious microorganisms
- Give three (3) examples of enriched media
- Culture media that contain nutrients that allow a particular group of organisms that might not be present in sufficient numbers in the specimen to be isolated and identified
- Give an example of Enrichment mediaand its use
- Culture media that contain ingredients that inhibit the growth of certain bacteria while permitting the growth of others
- An example of selective media and its use
- Culture media that allow colonies of one kind of organism to be distinguished from another. The media usually have constituents that cause observable changes in them when a particular biochemical reaction occurs
- Give an example of Differential media and its use
- Culture media that can not be heated are sterilized through what process?
- Membranes with pore diameters of 0.45μ are usually used for what?
- Membranes with pore diameters of 0.2μ are usually used for what?
- Most culture media that are heat stable are sterilized using what?
- What is the sufficient temperature to effect sterilization in autoclaving?
- What should be applied to reach the sufficient temperature in autoclaving?
- How much exposure time should be given to bigger volumes of materials?
- What indicators are used for sterilization?
- The sterilization basket should be placed at the _____, while the decontamination basket should be placed at the _____.
- Nutrient agar consists of what elements?
- Does agar affect the growth of media? Why?