Genetic Engineering

• Genetic engineering is the artificial manipulation, modification, and recombination of DNA
• Genetic Engineering is the process of using DNA technology to alter the genetic makeup of an organism.
• Genetic Engineering sometimes called biotechnology.
• Classical Breeding is the practice of mating or breeding.
• 1st step in Classical Breeding: Determine which trait are significant enough to be chosen.
• 2nd step in Classical Breeding: Select parents that exemplify these traits.
• 3rd step in Classical Breeding: Choose the best offspring from the parents to produce the next generation.
• 4th step in Classical Breeding: Repeat the process.
• The modification in genetic engineering: introduction of new traits into an organism,
• The modification in genetic engineering: enhancement of a present trait by increasing the expression of the desired gene, or
• The modification in genetic engineering: enhancement of the present trait by disrupting the inhibition of the desired gene's express
• 2 thing to create a recombinant DNA: 1. DNA containing the "gene of interest". and 2. Bacterial plasmid.
• 1st step in creating recombinant dna: Isolate DNA from two sources.
• 2nd step creating recombinant dna: cut both DNAs by the "restriction enzyme".
• 3rd step in creating recombinant DNA: combine the DNAs and seal using "DNA ligase".
• 4th step in creating recombinant DNA: transfer the recombinant DNA plasmid into the bacterial cell.
• 5th step in creating recombinant DNA: Isolation and insertion to other organisms to confer the desired trait.